This page is best viewed in Firefox.
Methods
EF5 Procedure
Materials
10x PBS
1000x Poisons
4% Paraformaldehyde
1X PBS
1X ttPBS
10mM EF5
Competed ttPBS
Antibody Carrier
Block
Normal Saline
Hoechst 33342
5-bromo-2’deoxyuridine
Peroxidase Reagents
ELK series Antibodies
In Vivo Use
ELK series Antibodies
- ELK series Antibodies
- Antibodies are stored frozen as concentrate in TTPBS with 20% glycerin.
- They come in the following conjugation flavors: plain vanilla, biotin, Cy3, Cy5, Alexa 488.
- When thawed, they are stable for many months.
- ELK3-51 is available as a concentrate, or diluted and ready to use (working strength is 75µg/ml).
- Competed antibody is only available at working strength.
- To make either from concentrate:
- Regular stain: 40µl of Ab concentrate + 960µl of Ab-carrier.
- Competed stain: 40µ of Ab conc. + 50µl of 10mM EF5 + 910µl of Ab carrier.
Comments? Questions? E-mail kochc@mail.med.upenn.edu