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Methods
EF5 Procedure
Materials
10x PBS
1000x Poisons
4% Paraformaldehyde
1X PBS
1X ttPBS
10mM EF5
Competed ttPBS
Antibody Carrier
Block
Normal Saline
Hoechst 33342
5-bromo-2’deoxyuridine
Peroxidase Reagents
ELK series Antibodies
In Vivo Use
1000x Poisons
- Azide
- 1.3g Azide in 10ml of water.
- 100mM Thimerosal
- 0.4g Thimerosal in 10ml of water.
Comments? Questions? E-mail kochc@mail.med.upenn.edu